- Determination of isoelectric point manual#
- Determination of isoelectric point software#
- Determination of isoelectric point iso#
- Determination of isoelectric point download#
This protocol specifies minimum and maximum pH, number of points, direction of pH change.
Determination of isoelectric point software#
Software controls their functions according to the preliminary defined protocol. Precision of the injection is 1 microliter. These devices are capable of injecting chemicals into the sample when it is stirred. Generally, determination of isoelectric point requires automatic burettes. Isoelectric point of rutile is around pH=3.8, whereas for alumina it is pH=9.3 Titration pH titration of concentrated alumina and rutile slurries. Examplesįigure below illustrates results of pH titrations for alumina slurry at 4% vl and rutile slurry at 7% vl. Consequently, this is an absolute definition of the isoelectric point. Phase of electroacoustic signal rotates 180 degrees at this pH value. of Dispersion Science and Technology, 23, 5, 601-617 (2002)Įvidently, this study established that our instruments determine isoelectric point with accuracy of 0.1 pH unit.ĭetermination of the isoelectric point does not require calibration of the electroacoustic device. “ Analysis of the Isoelectric Point in Moderately Concentrated Alumina Suspensions Using Electroacoustic and Streaming Potential Methods“, J. There was detail study of various methods suitable for characterizing iso-electric points conducted by international group: Hackley, V., Patton, J., Lum, L., Wasche, R.J., Naito, M., Abe, H., Hotta, Y., and Pendse, H. Precision of zeta potential measurements with models DT-310 and DT-1202 is ± 0.1 mV. Consequently, Electroacoustic method has big advantage over other methods of zeta potential characterization. Precision of the zeta potential measurement is critical for achieving the high accuracy of the isoelectric point determination. To determine this value, average the two pK a values that flank the neutral species. Colloidal systems – Methods for Zeta potential determination provides definition of the iso-electric point. The pH at which the net charge of an amino acid is zero is called the isoelectric point, or the pI.
Determination of isoelectric point iso#
Furthermore, ISO Standard 13099, Parts 1 and 3: 2012. There is semi-empirical rule stating that system would be stable when its pH located beyond 2 pH units vicinity of the iso-electric point. Stability of many dispersions and emulsions are often related to the pH of the solution.
Determination of isoelectric point manual#
This set-up allows users to determine zeta potential at a particular pH and then automatically repeat the measurement for the next pH in the series.īy minimising manual labour requirements using the BI-ZTU in combination with the Nanobrook zeta potential analyser the cost of isoelectric point determination is greatly reduced.Isoelectric point is a value of pH when zeta potential of the system equals 0. Using an autotitrator enables automatic addition of acid or base to adjust the pH of the sample, recording of pH, and loading of sample into the electrode cell of a Nanobrook zeta potential analyser.
Determination of isoelectric point download#
Incorporating four pumps the autotitrator provides high flexibility for optimising reagent use. Download Table Determination of isoelectric point -Gelatin 2 1 gelatin solution in water (powder gelatin) from publication: Validation of method for determining the isoelectric point of protein. The BI-ZTU autotitrator option for NanoBrook zeta potential analysers is ideal for automatic determination of the isoelectric point of colloids, for the detection of the onset of aggregation as a function of pH and for measure effect of salt concentration (ionic strength) on zeta potential. By studying the isoelectric point, scientists can determine how pH affects zeta potential and therefore can determine at which pH the zeta potential is zero. 04 November 2010 Contact sales When studying the stability of particle dispersions, the iso-electric point is an important characteristic. Hence, it is important to determine how pH affects the zeta potential of a dispersion. However, the surface chemistry of solid particles in a dispersion can be modified by altering parameters such as pH, surfactant concentration and salt concentrations. The stability of a dispersion is commonly determined by zeta potential. Testa Analytical Solutions reports on how automation of isoelectric point measurement can be achieved using a BI-ZTU autotitrator in combination with a Nanobrook zeta potential analyser.
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